Volume 9 Research
Chapter 0106 Policy for Testing of Biologicals Injected into Laboratory Animals
Responsible Office: Laboratory Animal Services
Originally issued: 10/27/2006
Biological materials must be determined to be free of contamination with agents of concern before use in rodents.
Reason For Policy
Animal cells and tissues can transmit pathogens and infect laboratory rodents. In order to protect laboratory animals housed at the Medical College of Georgia animal facilities, cells and tissues at risk should be tested for pathogens before they are injected or implanted into animals.
Entities Affected By This Policy
All researchers using laboratory animals for research purposes at the Medical College of Georgia and who will be injecting rodents with biological materials are affected by this policy.
Who Should Read This Policy
Faculty, staff, students, post-doctoral fellows, research assistants and associates, and any other personnel involved with using animals in research should know and observe this policy.
|Director, Laboratory Animal Services||706-721-3421||http://www.mcg.edu/research/animal/|
Printable Version of This Policy
Bhatt, P.N., et al. Viral and Mycoplasmal Infections of Laboratory Rodents: Effects on Biomedical Research. Academic Press, Orlando 1986.
Bhatt, P. N., Jacoby R.O. and S. W. Barthold. Contamination of transplantable murine tumors with lymphocytic choriomeningitis virus. Lab Anim Sci 36: 136-9, 1986.
Biggar, R.J., et al. LCM in laboratory personnel exposed to hamsters inadvertently infected with LCMV. JAVMA 1977; 171(9): 827-32.
Collins MJ, Parker JC. Murine virus contaminants of leukemia viruses and transplantable tumors. J Natl Cancer Inst 49: 1139-43, 1972.
Henderson KS, Banu LA, Havens RB, Jennings SM. Development of a 5 inch nuclease fluorogenic reverse transcriptase (RT) polymerase chain reaction (PCR) for the detection of Mouse Hepatitis Virus (MHV) in rodents and biological materials [Abstract] Cont Top Lab Anim Sci 38(4): 41, 1999.
Jacoby RO, Lindsey JR. Risks of infection among laboratory rats and mice at major biomedical research institutions. ILAR J 39(4): 266-71, 1998.
Lindsey JR, et al. Infectious Diseases of Rats and Mice, NRC, 1991.
Lipman NS. Mousepox: A threat to US mouse colonies. Lab Animal 28(6): 15, 1999.
Loew FM, Fox JG. Animal health surveillance and delivery systems. In: The Mouse in Biomedical Research. Vol III. Normative Biology, Immunology and Husbandry (Foster HL, Small JD, Fox JG, eds.), Academic Press, pp. 69-82, 1983.
Nicklas W, Kraft V, and Meyer B. Contamination of transplantable tumors, cell lines, and monoclonal antibodies and rodent viruses. Lab Anim Sci 43: 296-300, 1993.
Riley LK, Carty AJ, Besch-Williford CL. PCR-based testing as an alternative to MAP testing [Abstract] Cont Top Lab Anim Sci 38(4): 41, 1999.
Russell SP, Riley LK. PCR-based environmental monitoring for the detection of pathogenic organisms in rodents [Abstract] Cont Top Lab Anim Sci 38(4): 41, 1999.
These definitions apply to these terms as they are used in this policy:
|Biologicals||Biological materials such as cell lines, tissue homogenates, tumors, hybridomas, and blood products|
Biological material and animal products such as cell lines, tissues, and tumors have been repeatedly incriminated as vehicles for the introduction of animal pathogens into animal colonies.
The prevention of accidental introduction of viral infections into research animals is essential to maintenance of the animal colonies at the Medical College of Georgia. To maintain the integrity of the animal colonies Laboratory Animal Services (LAS) has established the guidelines described below regarding the introduction of cells, tumors, and other biological products into experimental animals.
For screening biological materials, such as transplantable tumors, cell lines, embryonic stem cells, hybridomas, blood products, and tissue homogenates:
- Screening must be documented and added to the Animal Use Protocol before an Approval Letter is awarded.
- If the investigator can provide a report (within 3 months) that the material is free of rodent pathogens, the biological material will not need to be retested.
- Biological samples must be screened by either PCR or an equivalent method for rodent pathogens prior to use in rodents if the source of the material is of unknown pathogen status (i.e., acquired or developed from animals housed at an outside institution or from an outside vendor who does not screen for rodent pathogens).
- The frequency of retesting and the number of pathogens to be screened will be determined on how often the biological sample is utilized, suspicion of contamination, and source of biological material.
- For biological materials currently being used by investigators, the pathogens to be tested for will be based on the pathogens of contamination within the animal facility, i.e., mouse parvovirus and mouse hepatitis virus.
- For biological materials that will be imported from an outside source (e.g., other institution, or vendor), the following pathogens must be screened for, if proof of screening can not be provided:
- Mycoplasma sp.
- Sendai virus
- Mouse hepatitis virus (MHV)
- Pneumonia virus of mice
- Mice minute virus
- Mouse parvovirus (MPV)
- Theilers murine encephalomyelitis
- The biological samples to be tested should be submitted to the LAS veterinary technician for submission to a reputable diagnostic laboratory. The samples will be packaged according to the diagnostic laboratory’s specifications.
- The cost of screening will be at the expense of the investigator.
The responsibilities each party has in connection with Academic, Research, and Student Affairs Policy 9.0106, Policy for Testing of Biologicals Injected into Laboratory Animals, are:
|Principal Investigator (PI)||Provide LAS with suitable documentation of the specimen’s source, history of use, and any previous testing to allow LAS to determine whether the specimen is likely to be free of all microbial agents of concern or it requires further testing.|
|LAS staff||Assess testing methods and results as provided by the PI and determine whether specimens to be injected or implanted into rodents are most likely free of rodent pathogens. LAS will provide specific recommendations for approved methods and laboratories and will assist the PI in arranging the required testing.|
|IACUC||Is responsible for the periodic review, evaluation, and enforcement of this policy.|